The synthesis of acidic nuclear proteins in the prereplicative phase of the isoproterenol-stimulated salivary gland.
نویسندگان
چکیده
A single injection of isoproterenol results, after a lag period of 20 hours, in a marked stimulation of DNA synthesis and cell proliferation in the mouse salivary gland. The incorporation of leucine-3H into three acidic nuclear protein fractions (nonhistone nuclear proteins) increases within 30 min following the administration of isoproterenol and does not return to control levels until 48 hours later. The three fractions consist of (a) a fraction soluble in Tris-EDTA (ribonucleoprotein fraction) ; (b) one extractable from nuclei with 0.15 N NaCl; and (c) an acidic fraction insoluble in the above solutions and in 0.25 N H&O4 (residual fraction). The incorporation of leucine-3H into acidic nuclear proteins reaches its peak 12 hours after isoproterenol injection while the peak activity of the histone fraction coincides with the peak of DNA synthesis. Since the specific activity of the leucine pool does not change in stimulated glands, the increased incorporation of leucine-3H into acidic nuclear proteins indicates an increase in the rate of synthesis of these proteins. Actinomycin D given 30 min prior to isoproterenoi-irili&Xs isoproterenol-stimulated DNA synthesis but does not affect the increase in the rate of synthesis of nuclear proteins which occurs 2 hours after isoproterenol. However, the increases in the rates of synthesis occurring at 8 and 12 hours are inhibited. Cycloheximide, administered 1 hour after isoproterenol, inhibits isoproterenol-stimulated DNA synthesis as well as the increase in acidic nuclear protein synthesis at 8 and 12 hours. These results indicate a temporal difference in the synthesis of histones and acidic proteins during the transition of Go cells to the S phase and suggest that acidic nuclear proteins may be involved in the control of cell proliferation in mammalian cells.
منابع مشابه
The Synthesis of Acidic Nuclear Proteins in the Prereplicative Phase of the Isoproterenoll-stimulated Salivary Gland*
A single injection of isoproterenol results, after a lag period of 20 hours, in a marked stimulation of DNA synthesis and cell proliferation in the mouse salivary gland. The incorporation of leucine-3H into three acidic nuclear protein fractions (nonhistone nuclear proteins) increases within 30 min following the administration of isoproterenol and does not return to control levels until 48 hour...
متن کاملEffect of irradiation on mouse salivary glands during the prereplicative phase of isoproterenol-stimulated DNA synthesis.
When mice were given a single injection of isoproterenol (IPR), the salivary gland cells were stimulated to enter DNA synthesis in a fairly synchronous manner after a lag period of about 18 hr. 7-ray irradiation of these cells in the early prereplicative phase effectively inhibited the stimulation of DNA synthesis caused by IPR. However, irradiation in the late prereplicative phase or in mid-DN...
متن کاملEffect of Irradiation on Mouse Salivary Glands during the Prereplicative Phase of Isoproterenol-stimulated DNA Synthesis1
When mice were given a single injection of isoproterenol (IPR), the salivary gland cells were stimulated to enter DNA synthesis in a fairly synchronous manner after a lag period of about 18 hr. 7-ray irradiation of these cells in the early prereplicative phase effectively inhibited the stimulation of DNA synthesis caused by IPR. However, irradiation in the late prereplicative phase or in mid-DN...
متن کاملthe effect of isoproterenol on protein electrophoretic pattern of rabbit salivary Glands
Isoproterenol is a potent B-adrenergic agonist which its effect on rabbit salivary Glands has been studied in this research.two groups of male albino rabbits(five in each group with the mean weight of 1000+_300 gr) were injected with 20 mg/kg of isoproterenol intrapritoneally each day for fifteen days and one similar group(n=5) was chosen as control group.in one of the test groups in addition ...
متن کاملImproved quantification of salivary gland scintigraphy by means of factor analysis
Introduction: In this study the automatic separation of oral and salivary gland activity and spontaneous secretion by means of factor analysis for quantitative salivary gland scintigraphy is introduced. Methods: After intravenous administration of 99mTc sodium pertechnetate, dynamic scintigraphy was performed. 20 minutes after tracer application 2 ml of lemon juice was delivered to stimulate t...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 245 22 شماره
صفحات -
تاریخ انتشار 1970